206 research outputs found

    Fear of Social Isolation: Testing an Assumption from the Spiral of Silence

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    This study is designed specifically to test the fear of isolation assumption and to explore its position in the Spiral of Silence model. It has been unclear from the literature whether fear of isolation is antecedent to opinion formation and dominant opinion assessment or an intervening variable between opinion formation and willingness to voice the opinion. Path analyses are used to empirically investigate the relationship of the fear of isolation variable to other variables in the model

    Readers as gatekeepers of online news: Brazil, China, and the United States

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    What is news? News values revisited (again)

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    The deceptively simple question “What is news?” remains pertinent even as we ponder the future of journalism in the digital age. This article examines news values within mainstream journalism and considers the extent to which news values may be changing since earlier landmark studies were undertaken. Its starting point is Harcup and O’Neill’s widely-cited 2001 updating of Galtung and Ruge’s influential 1965 taxonomy of news values. Just as that study put Galtung and Ruge’s criteria to the test with an empirical content analysis of published news, this new study explores the extent to which Harcup and O’Neill’s revised list of news values remain relevant given the challenges (and opportunities) faced by journalism today, including the emergence of social media. A review of recent literature contextualises the findings of a fresh content analysis of news values within a range of UK media 15 years on from the last study. The article concludes by suggesting a revised and updated set of contemporary news values, whilst acknowledging that no taxonomy can ever explain everything

    A Tale of Two Stories from "Below the Line": Comment Fields at the Guardian

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    This article analyzes the nature of debate on “below the line” comment fields at the United Kingdom’s Guardian, and how, if at all, such debates are impacting journalism practice. The article combines a content analysis of 3,792 comments across eighty-five articles that focused on the UN Climate Change Summit, with ten interviews with journalists, two with affiliated commentators, plus the community manager. The results suggest a more positive picture than has been found by many existing studies: Debates were often deliberative in nature, and journalists reported that it was positively impacting their practice in several ways, including providing new story leads and enhanced critical reflection. However, citizen–journalist debate was limited. The results are attributed to the normalization of comment fields into everyday journalism practice, extensive support and encouragement from senior management, and a realization that comment fields can actually make the journalists’ life a little easier

    Global Identification and Characterization of Transcriptionally Active Regions in the Rice Genome

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    Genome tiling microarray studies have consistently documented rich transcriptional activity beyond the annotated genes. However, systematic characterization and transcriptional profiling of the putative novel transcripts on the genome scale are still lacking. We report here the identification of 25,352 and 27,744 transcriptionally active regions (TARs) not encoded by annotated exons in the rice (Oryza. sativa) subspecies japonica and indica, respectively. The non-exonic TARs account for approximately two thirds of the total TARs detected by tiling arrays and represent transcripts likely conserved between japonica and indica. Transcription of 21,018 (83%) japonica non-exonic TARs was verified through expression profiling in 10 tissue types using a re-array in which annotated genes and TARs were each represented by five independent probes. Subsequent analyses indicate that about 80% of the japonica TARs that were not assigned to annotated exons can be assigned to various putatively functional or structural elements of the rice genome, including splice variants, uncharacterized portions of incompletely annotated genes, antisense transcripts, duplicated gene fragments, and potential non-coding RNAs. These results provide a systematic characterization of non-exonic transcripts in rice and thus expand the current view of the complexity and dynamics of the rice transcriptome

    Transethnic Genome-Wide Association Study Provides Insights in the Genetic Architecture and Heritability of Long QT Syndrome

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    BACKGROUND: Long QT syndrome (LQTS) is a rare genetic disorder and a major preventable cause of sudden cardiac death in the young. A causal rare genetic variant with large effect size is identified in up to 80% of probands (genotype positive) and cascade family screening shows incomplete penetrance of genetic variants. Furthermore, a proportion of cases meeting diagnostic criteria for LQTS remain genetically elusive despite genetic testing of established genes (genotype negative). These observations raise the possibility that common genetic variants with small effect size contribute to the clinical picture of LQTS. This study aimed to characterize and quantify the contribution of common genetic variation to LQTS disease susceptibility. METHODS: We conducted genome-wide association studies followed by transethnic meta-analysis in 1656 unrelated patients with LQTS of European or Japanese ancestry and 9890 controls to identify susceptibility single nucleotide polymorphisms. We estimated the common variant heritability of LQTS and tested the genetic correlation between LQTS susceptibility and other cardiac traits. Furthermore, we tested the aggregate effect of the 68 single nucleotide polymorphisms previously associated with the QT-interval in the general population using a polygenic risk score. RESULTS: Genome-wide association analysis identified 3 loci associated with LQTS at genome-wide statistical significance (P&lt;5×10-8) near NOS1AP, KCNQ1, and KLF12, and 1 missense variant in KCNE1(p.Asp85Asn) at the suggestive threshold (P&lt;10-6). Heritability analyses showed that ≈15% of variance in overall LQTS susceptibility was attributable to common genetic variation (h2SNP 0.148; standard error 0.019). LQTS susceptibility showed a strong genome-wide genetic correlation with the QT-interval in the general population (rg=0.40; P=3.2×10-3). The polygenic risk score comprising common variants previously associated with the QT-interval in the general population was greater in LQTS cases compared with controls (P&lt;10-13), and it is notable that, among patients with LQTS, this polygenic risk score was greater in patients who were genotype negative compared with those who were genotype positive (P&lt;0.005). CONCLUSIONS: This work establishes an important role for common genetic variation in susceptibility to LQTS. We demonstrate overlap between genetic control of the QT-interval in the general population and genetic factors contributing to LQTS susceptibility. Using polygenic risk score analyses aggregating common genetic variants that modulate the QT-interval in the general population, we provide evidence for a polygenic architecture in genotype negative LQTS.</p
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